Morphology of Grammatophyllum specinocum BL. after Paclobutrazol Treatment in Sterile Conditions
Keywords:
Paclobutrazol, plant morphology, orchids, plant tissue cultureAbstract
Paclobutrazol (PBZ) is a plant hormone in the group of plant growth retardants that has been applied to plants in sterile conditions. The purpose is to encourage plants to have different morphological characteristics. Therefore, in this study, PBZ was applied to the protocorm-like bodies (PLBs) of the Tiger orchid. PBZ solution at concentrations of 0, 20, 40 and 60 mg/l were added to MS medium with 1 mg/l BA, 3% sucrose and 0.75% agar and the explants were cultured for 4 weeks. It was found that higher PBZ concentrations resulted in survival and shoot formation decreased statistically significant difference (P≤0.01). The PBZ concentration of 49.22 mg/l caused the death of 50% of PLBs. The morphology of orchids grown in a PBZ-free medium showed normal growth. Elongated shoots are green with an average height of 10.30 cm. The seedlings in the PBZ medium had shorter stems than the control. At the concentration of 60 mg/l PBZ, the shortest orchid stem was 3.78 cm, and the lowest root formation of 57.14%. However, there was no difference in several roots in each experiment. The seedlings had a 100% survival rate after acclimatization. After hardening, plants derived from PBZ-free medium had elongated, slender leaves, while seedlings grown on PBZ medium had short, thick stems, large leaves, and dark green color. The orchid plants can continue to grow normally after transplanting into the soil.
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