Morphology of Grammatophyllum specinocum BL. after Paclobutrazol Treatment in Sterile Conditions
Keywords:
Paclobutrazol, plant morphology, orchids, plant tissue cultureAbstract
Paclobutrazol (PBZ) is a plant hormone in the group of plant growth retardants that has been applied to plants in sterile conditions. The purpose is to encourage plants to have different morphological characteristics. Therefore, in this study, PBZ was applied to the protocorm-like bodies (PLBs) of the Tiger orchid. PBZ solution at concentrations of 0, 20, 40 and 60 mg/l were added to MS medium with 1 mg/l BA, 3% sucrose and 0.75% agar and the explants were cultured for 4 weeks. It was found that higher PBZ concentrations resulted in survival and shoot formation decreased statistically significant difference (P≤0.01). The PBZ concentration of 49.22 mg/l caused the death of 50% of PLBs. The morphology of orchids grown in a PBZ-free medium showed normal growth. Elongated shoots are green with an average height of 10.30 cm. The seedlings in the PBZ medium had shorter stems than the control. At the concentration of 60 mg/l PBZ, the shortest orchid stem was 3.78 cm, and the lowest root formation of 57.14%. However, there was no difference in several roots in each experiment. The seedlings had a 100% survival rate after acclimatization. After hardening, plants derived from PBZ-free medium had elongated, slender leaves, while seedlings grown on PBZ medium had short, thick stems, large leaves, and dark green color. The orchid plants can continue to grow normally after transplanting into the soil.
References
Abdalla, N., Taha, N., Bayoumi, Y., El-Ramady, H. and Shalaby, T.A. 2021. Paclobutrazol applications in agriculture, plant tissue cultures and its potential as stress ameliorant: a mini-review. Environment, Biodiversity and Soil Security 5: 245-257.
Bueno, P.M.C., Tofanelli, M.B.D., Vendrame, W.A. and Biasi, L.A. 2021. Paclobutrazol as an alternative to improve propagation of Rubus brasiliensis Mart. Scientia Horticulturae 287(2): 110215 DOI:10.1016/j.scienta.2021.110215.
Chatsuwan, Y. and Rungruchkanont, K. 2018. Effects of media composition and light condition germination and development of Grammatophyllum speciosum Blume seed in vitro. The 19th National Graduate Research Conference March 9, 2018, at Khon Kaen University.
Choopeng, S. and Te-chato, S. 2019. Effect of plant growth regulators on growth and in vitro flowering of hybrid between Dendrobium santana and D. friedericksianum. Songklanakarin Journal of Plant Science 6(1): 38-47.
Chowjarean, V., Sucontphunt, A., Vchirawongkwin, S., Charoonratana, T., Songsak, T., Harikarnpakdee, S. 2018. Validated RC-HPLC method for quantification of gastrin in ethanolic extract from the pseudobulbs of Grammatophyllum speciosum Blume,” Malaysian Journal of Analytical Science 22(2): 219– 226.
Desta, B. and Amare, G. 2021. Paclobutrazol as a plant growth regulator. Chemical and Biological Technologies in Agriculture 8(1): 1-15.
Gaston, M.L. 2001. Tips on regulating the growth of floriculture crops. United States: O.FA. Services Incorporated.
Gimenes, R., Pivetta, Kathia F.L., Mazzini-Guedes, R.B., Ferraz, M.V., Pereira, S.T.S., Santos, Á.S., Tadeu de Faria, R. and Praseres de Almeida, L.C. 2018. Paclobutrazol on in vitro growth and development of Zygopetalum crinitum Orchid, and on seedling acclimatization. American Journal of Plant Science 9: 1029-1036.
Hongpakdee, P. 2014. Effects of paclobutrazol on water use and growth of potted marigolds. Journal of Agriculture 30(3): 281-289.
Jiropasphanuwong, Y., Ramasoot, S., Mahannop, K. and Ninkrawat, T. 2015. Enhancement of Exacum affine Balf. tissue culture in vitro by using paclobutrazol. Wichcha Journal 34(1): 53-60.
Kumar, G., Lal, S., Bhatt, P., Ram, R.A., Bhattacherjee, A.K., Dikshit, A., and Rajan, S. 2021. Mechanisms and kinetics for the degradation of paclobutrazol and biocontrol action of a novel Pseudomonas putida strain T7. Pesticide Biotechnology and Physiology 175: 1-10.
Muangkaewngam, A. and Te-chato, S. 2016. Effect of paclobutrazol on in vitro culture of Torch Ginger (Etlingera elatior [Jack] R.M.Smith.). Princess of Naradhiwas University Journal 8(1): 111-116.
Obsuwan, K., Deesubin, P., Tongam, A. and Juneenat, O. 2021. Influence of paclobutrazol on growth of Dendrobium ‘Sonia Jo Daeng’ under salt stress condition in tissue culture. Science, Engineering and Health Studies 15, 21030001. https://doi.org/ 10.14456/sehs.2021.3.
Pattanachatchai, N. 2014. Paclobutrazol: effects on canopy growth and chlorophyll content of Adenium obesum cv. Holland. Khon Kaen Agriculture Journal 42(1): 39-46.
Phengchang, P., Suvittawat, K., Tanongjid, K., Thawornchareon, D. and Saradhuldhat, P. 2022. Effect of paclobutrazol on pseudostem sucker growth of Musa (AAA group) ‘Kluai Hom Thong’. Journal of Agricultural Science and Management 5(1): 13-18.
Promchan, T., Harattaya, K., Rungsawang, P., Tabtim, A. and Meetum, P. 2015. Effect of applied volume of paclobutrazol on growth and flowering of American hybrids marigold. Wichcha Journal 34(1): 26-37.
Saenthongkham, A., Sumana, N., Jogloy, S. and Hongpakdee, P. 2019. Effects of PBZ and MPC for control of growth and flowering of potted Jerusalem Artichoke (Helianthus tuberosus L.). King Mongkut’s Agricultural Journal 37(2): 200-211.
Samala, S., Te-chato, S., Yenchon, S. and Thammasiri, K. 2014. Protocorm-like body proliferation of Grammatophyllum speciosum through a symbiotic seed germination. ScienceAsia 40: 379-383.
Tauk, C., Chuaduangpui, P. and Khawniam, T. 2021. Effect of explants on plant regeneration and concentration of paclobutrazol on morphological response of Dwarf Water Hyssop (Bacopa monnieri). Naresuan University Journal: Science and Technology 29(3): 56-66.
Uawonggul, N., Chaveerach, A., Thammasirirak, S., Arkaravichien, T., Chua, C. and Daduang, S. 2006. Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis. Journal of Ethnopharmacology 103(2): 201-207.
Wen, Z.Z., Lin, Y., Lui, Y.Q., Wang, M., Wang, Y.Q. and Liu, W. 2013. Effects of paclobutrazol in vitro on transplanting efficiency and root tip development of Dendrobium nobile. Biologia Plantarum 57(3): 576-580.
Wiriyananont, Y., Krajang, N. and Samitthiarporn, S. 2019. Effect of paclobutrazol on morphological characteristics of Cymbidium finlaysonianum Lindl. in vitro. Songklanakarin Journal of Plant Science 6(4): 19-24.
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