Morphological Characteristics, Hydrogen Peroxide Content and Antioxidant Enzyme Activity in Rubber Homograft and Heterograft
Keywords:
Para rubber, Rootstock, Scion, Compatibility, Reactive oxygen speciesAbstract
Plant propagation is one of processes to increase plant number and to be used for plant genetic conservation. Para rubber is propagated by many methods such as seeds, cleft grafting or budding. Budding is widely and effectively used for para rubber tree propagation. However, there could be problems with graft compatibility between rootstock and scion. The grafting healing process may trigger oxidative stress. Plants have mechanisms for producing antioxidants to prevent damage of plant tissues at budding sites. The aim of this study was to investigate morphological and biochemical responses of budded para rubbers by comparing the homograft budding (RRIM 600 / RRIM 600) with heterograft budding (RRIM 600 / PB5/51) of rubber. The results showed that heterograft gave a higher percentage of budding success than homograft at 81.58% and shorter new shoot formation times at 6 days. The higher average new-shoot height, canopy diameter and average number of leaves were obtained from homograft. RRIM 600 / RRIM 600 was likely to increase H2O2 while RRIM 600 / PB5/51 showed the increasing H2O2 after 3-7 days of budding but greatly reduced after 14-21 days. In addition, RRIM 600 / RRIM 600 showed the decrease of SOD APX CAT and POD activities while RRIM 600 / PB5/51 showed the increase of APX, CAT and POD activities. Therefore, the compatibility between scion and rootstock could possibly affect morphological and biochemical responses.
References
Abbas, B.S. and Ginting, S. 1981. Influence of rootstock and scion on girth increment in rubber trees. Buletin Balai Penelitian Perkebunan Medan. 12: 145-152.
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology 105: 121–126.
Aloni. B., Karni, L., Deventurero, G., Levin, Z., Cohen, R., Katzir, N., Lotan-Pompan, M., Edelstein, M., Aktas, H., Turhan, E., Joel, D.M., Hoerv, C. and Kapulnik, Y. 2008. Physiological and biochemical changes at the rootstock-scion interface in graft combinations between Cucurbita rootstocks and a melon scion. Journal of Horticultural Science and Biotechnology 83: 777-783.
Beyer, W.F. and Fridovich, I. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161: 559–566.
Chen, Z., Zhao, J., Qin, Y. and Hu, G. 2016. Study on the graft compatibility between ‘Jingganghongnuo’ and other litchi cultivars. Scientia Horticulturae 199: 56-62.
Cherngchalard, K. 2012. Selection of Rubber Clones for Rootstock and Genetically Analysis Using DNA Markers. Master Thesis. Prince of Songkla University.
Dias, M.A. and Costa, M.M. 1983. Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. Journal of Experimental Botany 34: 537–543.
Fernández-garcía, N., Carvajal, M. and Olmos, E. 2004. Graft union formation in tomato plants: peroxidase and catalase involvement. Annals of Botany 93: 53-60.
Gainza, F., Opazo, I. and Muñoz, C. 2015. Graft incompatibility in plants: metabolic Changes during formation and establishment of the rootstock/scion union with emphasis on Prunus species. Chilean Journal of Agricultural Research 75: 28-34.
Goncalves, P.S. and Martins, A.L.M. 2002. Combining ability effects of clonal rootstocks and scions in rubber trees (Hevea). Crop Breeding and Applied Biotechnology 3: 445-452.
Irisarri, P., Binczycki, P., Errea, P., Martens, H.J. and Pina, A. 2015. Oxidative stress associated with rootstock-scion interactions in pear/quince combinations during early stages of graft development. Journal of Plant Physiology 176: 25-35.
Jantarapratin, A., Sittiphol, B., Pattanakul, P., Janruang, N. and Suppaphol, P. 1995. Screening for resistance to white root disease of rubber stock. In Full Report (ed. Jantarapratin, A.) pp. 30-39. Songkhla: Songkhla Rubber Research Center, Rubber Research Institute.
Junglee, S., Urban, L., Sallanon, H. and Lopez-Lauri, F. 2014. Optimized assay for hydrogen peroxide determination in plant tissue using potassium iodide. American Journal of Analytical Chemistry 5: 730-736.
Kaveeta, L. 2016. Plant structure. Bangkok: Department of Botany, Faculty of Science Kasetsart University.
Kitajima, S. 2008. Peroxide-mediated inactivation of two chloroplastic peroxidase, ascorbate peroxidase and 2-Cys peroxiredoxin. Photochemistry and Photo-biology 84: 1404-1409.
León, J., Rojo, E. and Sánchez-Serrano, J. J. 2001. Wound signaling in plant. Journal of Experiment Botany 52: 1-9.
Łukazuk, E. and Ciereszko, I. 2012. Biological diversity – from cell to ecosystem. Białystok: Polish Botanical Society.
Martínez-Bellesta, M.C., Alcaraz-López, C., Muries, B., Mota-Cadenas, C. and Carvajal, M. 2010. Physiological aspects of rootstock-scion interactions. Scientia Horticulturae 127: 112-118.
Mittler, R., Vanderauwera, S., Gollery, M. and Breusegem, F.V. 2004. Reactive oxygen gene network of plants. TRENDS in Plant Science 9: 490-498.
Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–280.
Nakkanong, K. and Nualsri, C. 2014. Research of Expression of white root resistance related genes in rubber tree. Songkhla: Faculty of Natural Resources Prince of Songkla University.
Orozco-Cardenas, M. and Ryan, C.A. 1999. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proceedings of the National Academy of Sciences of the United States of America 96: 6553-6655.
Porika, H.K., Nimbolkar, P.K., Rajashekar, B. and Hussain, S. F. 2016. Graft compatibility-incompatibility in fruit crops: Mechanism and determination techniques. The Asian Journal of Horticulture 11: 252-260.
Prabpree, A. 2019. Exrpession Profile of Phenylpropanoid Related Genes on Compatibility of Rubber Tree Rootstock-scion. Master Thesis. Prince of Songkla University.
Rubber Research Institute. 2012. Data of Para Rubber. Bangkok: Rubber Division, Department of Agriculture.
Rubber Research Institute. 2016. Para Rubber Bulletin. Para Rubber Electronic Bulletin 25: 2-41.
Sangsanga, T. 2014. PGPR: The role in promoting and protecting plant under stress conditions. Thai Science and Technology Journal: 553-570.
Sangsil, P. 2015. The Relationship between PAL Gene Expression and Phenolic Compounds in Compatibility of Different Rubber Rootstock-scion. Master Thesis. Prince of Songkla University.
Sewelam, N., Kazan, K. and Schenk, P.M. 2016. Global plant stress signalling: reactive oxygen species at the cross-road. Frontiers in Plant Science 7: 1-21.
Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli, M. 2012. Reactive oxygen species, oxidative damage and antioxidant defenses mechanism in plants under stressful conditions. Journal of Botany 2012: 1-26.
Slesak, I., Slesak, H., Libik, M. and Miszalski, Z. 2008. Antioxidant response system in the short-term post-wounding effect in Mesembryanthemum crystallinum leaves. Journal of Plant Physiology 165: 127-137.
Songkhla Rubber Research Center and Suratthani Rubber Research Center. 2003. Production of Rubber in South. Bangkok: Rubber Division, Department of Agriculture, Ministry of Agriculture and Cooperatives.
Thongneaungam, O. 2007. Growing media and Preparation of Planting Material. Bangkok: Rubber Division, Department of Agriculture, Ministry of Agriculture and Cooperatives.
Trinchera, A., Pandozy, G.S., Rinaldi, P., Crinò, Temperini O. and Rea, E. 2013. Graft union formation in artichoke grafting onto wild and cultivated cardoon: an anatomical study. Journal of Plant Physiology 170: 1569-1578.
Vrčiš, S., Pulko, B. and Kocsis, L. 2015. Factors influencing grafting success and compatibility of grape rootstocks. Scientia Horticulturae 181: 168-173.
Wattanaphoot, N. 1999. Plant Propagation. Bangkok: OS printing house.
Wongvarodom, V. and Lerslerwong, L. 2013. Research of Preliminary Study for Development of Rubber Rootstock Seed Production. Songkhla: Faculty of Natural Resources Prince of Songkla University.
Xu, Q., Guo, S.R., Li, H., Du, N.S., Shu, S. and Sun, J. 2015. Physiological aspects of compatibility and incompatibility in grafted cucumber seedlings. Journal of the American Society for Horticultural Science 140: 299-307.
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